View sam file linux5/26/2023 ![]() ![]() We’ll be using some data and steps from the Genomics Virtual Lab Variant Detection tutorials. The first is Alignment using the Galaxy bioinformatics workflow environment, the second is Alignment using the Unix/Linux command line. You will perform the same analysis in both sections. In this tutorial we will be performing some alignments of short reads to a longer reference (as outlined in earlier lectures.) It is split into two sections. Molecular Dynamics - Building input files, visualising the trajectory Molecular Dynamics - Introduction to cluster computing Identifying proteins from mass spectrometry data RNAseq differential expression tool comparision (Galaxy) Introduction to Metabarcoding using Qiime2 Hybrid genome assembly - Nanopore and Illumina Introduction to de novo genome assembly for Illumina readsĭe novo assembly of Illumina reads using Velvet (Galaxy)ĭe novo assembly of Illumina reads using Spades (Galaxy) Introduction to de novo assembly with Velvet
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